Efficient soluble production of folded cat allergen Fel d 1 in Escherichia coli

The major cat allergen Fel d 1 is one of the most common and potent causes animal related allergy. Medical treatment allergy has relied on immunotherapy carried out with dander extract. This approach been problematic, mainly due to inconsistent levels in produced extracts. Recombinant DNA technology proposed as an alternative method produce more consistent pharmaceuticals for diagnostics Current approaches recombinant (recFel 1) cytoplasm Escherichia coli have however resulted protein folding deficiencies insoluble inclusion body formation, requiring elaborate vitro processing acquire folded material. In this study, we introduce efficient cytoplasmic production recFel that utilizes eukaryotic factors aid fold be soluble fraction E. coli. solubly expressed shown by biophysical experiments contain structural disulfides, extremely stable, a sensitivity methionine sulfoxidation. latter discussed context functional relevance.